Dorsa Sadigh, “Anatomical cell type mapping in the whole mouse brain and common coordinate framework”

/ August 19, 2022/

When:
November 1, 2022 @ 12:00 pm – 1:15 pm
2022-11-01T12:00:00-04:00
2022-11-01T13:15:00-04:00

“Anatomical cell type mapping in the whole mouse brain and common coordinate framework”

 

Yongsoo Kim, PhD

Associate Professor

Penn State University

 

Abstract: The brain contains multiple neuronal and non-neuronal cell types with distinct spatial relationship that serve as a basic building block in the brain. I previously developed high resolution mapping methods to visualize and to quantify these individual brain cell types across the entire mouse brain using 3D reference atlases. In this talk, I will present my lab’s effort to understand spatial arrangement of several major cell types including GABAergic neuronal cell types and oxytocin neurons. Moreover, I will discuss our new methods to trace the complete network of cerebrovasculature and to quantify perivascular pericytes across the mouse brain. In all my works, 3D reference brains play critical role to integrate individual mapping data into the standard anatomical framework. My lab established a new online anatomical atlas for the adult mouse brain that combines the segmentation of the Allen Mouse Brian Atlas and Paxinos Mouse Brain Atlas. We also developed 3D atlas frameworks for early postnatal brains. Furthermore, my lab is developing multimodal common coordinate frameworks from embryonic and early postnatal mouse brains. Using these resources, my lab is finely mapping spatiotemporal trajectories for GABAergic neurons in developing mouse brains. In summary, high resolution anatomical mapping with 3D atlases helps to unveil overall anatomical organizational plans of cell types across different brain areas.

 

Biography: Yongsoo Kim, PhD is an Associate Professor in the Department of Neural and Behavioral Sciences at Penn State University. Yongsoo Kim’s main research goal is to gain systematic understanding of anatomical and functional organization of different cell types in mammalian brains to support cognitive functions using mice as an animal model. Towards this goal, he utilizes high-resolution 3D imaging (Serial Two-photon Tomography; STPT and Light Sheet Fluorescence Microscopy; LSFM) and streamlined data processing to systematically analyze target signals in the entire mouse brain. Previously, he successfully applied this method to uncover a whole-brain map of activated regions representing different social behaviors in adult mice and further developed the mapping method to quantify the distribution of different GABAergic neuronal subtypes in the whole mouse brain (Kim et al., 2015; 2017). Recently, he established complete maps of cerebrovascular network including pericytes and identified strong brain regional correlation with specific neuronal subtypes (e.g., parvalbumin+ cortical interneurons) (Wu et al., 2022). Moreover, Yongsoo utilized his method to achieve mesoscale anatomical mapping in combination with several viral and genetic tools to establish comprehensive wiring diagram of oxytocin neurons in the mouse brain (Son et al., 2022). Yongsoo also has significant expertise in establishing digital 3D atlases for the mouse brain. He previously created an STPT based 3D atlas framework with Allen Reference Atlas labels (Kim et al., 2015). To systematically integrate different atlases in the same spatial framework, his lab established Franklin-Paxinos atlas based labels with detailed segmentations in the dorsal striatum onto the Allen common coordinate framework (CCF) (Chon et al., 2019). To share these large-scale imaging data, he utilizes web visualization for others in the scientific community to easily access and share the dataset.

Tuesdays, 12pm-1:15pm

Held virtually in person at Clark 110 & over Zoom

Check for event details: https://www.minds.jhu.edu/events/calendar/

 

 

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Meeting ID: 986 2441 3365

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Meeting ID: 986 2441 3365

 

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